Elicitation of Pterocarpan Phytoalexins in Cell Suspension Cultures of Different Chickpea (Cicer arietinum L.) Cultivars by an Elicitor from the Fungus Ascochyta rabiéi
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چکیده
Cell suspension cultures of two chickpea (Cicer arietinum L.) cultivars, resistant and susceptible towards the chickpea pathogen Ascochyta rabiei, were compared with regard to elicitor-induced changes in phytoalexin and isoflavone accumulation. The elicitor was isolated from fermentergrown mycelium of A. rabiei and it mainly consisted of glucose, mannose and N-acetylgalactosamin. Time course and dose response studies on elicitor action demonstrated that the cell culture of the resistant cultivar ILC 3279 accumulated large amounts of the pterocarpan phytoalexins medicarpin and maackiain within 8 h. The cell culture of the susceptible cultivar ILC 1929 accumulated only small amounts of the phytoalexins some 12 h after elicitor treatment. Growth of the cell cultures and the accumulation of isoflavones and isoflavone conjugates were not altered by elicitor treatment except for a higher accumulation of formononetin 7-0-glucoside-6"-0-malonate in cell culture ILC 3279 subsequent to the maximum of phytoalexin accumulation.
منابع مشابه
Elicitor Induction of Cytochrome P-450 Monooxygenases in Cell Suspension Cultures of Chickpea (Cicer arietinum L.) and Their Involvement in Pterocarpan Phytoalexin Biosynthesis
A yeast glucan elicitor causes the accumulation o f the pterocarpan phytoalexins medicarpin and maackiain in chickpea ( Cicer arietinum) cell suspension cultures established from seeds. A cell culture line from a chickpea cultivar resistant against its main fungal pathogen Ascochyta rabiei accumulates large amounts (944 nmol/g fr. wt.) whereas a cell culture line from a susceptible cultivar acc...
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